Separation/Analysis of Chrysophanol by Ionic Liquid Loaded Β-Cyclodextrin Polymer Coupled with Ultraviolet Spectrophotometry

Chrysophanol (Chry, Figure 1), 1,8-dihydroxy-3methylanthraquinone, is a free anthraquinone compound and a secondary metabolite of medicinal plant rhubarb. Chry has been reported to have many pharmacological effects such as anti-inflammatory activity [1], anti-microbial activity [2] and anti-cancer action [3]. Therefore, the detection of Chry is quite important and interesting. Until now, various techniques have been developed for the determination of Chry to date, including high-performance liquid chromatography [4] and fluorescence spectrophotometry [5]. However, the direct determination with spectrophotometry is difficult owing to matrix effects and low concentration of Chry in samples. Therefore, the necessity of separation/preconcentration procedure of Chry is significant (Figure 1).